Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster

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dc.contributor.author Drolia, Rishi
dc.date.accessioned 2012-06-07T15:04:08Z
dc.date.available 2012-06-07T15:04:08Z
dc.date.issued 2012-06-07
dc.identifier.uri http://hdl.handle.net/2097/13920
dc.description.abstract Ehrlichia chaffeensis is a Gram-negative, obligatorily intracytoplasmic bacterium and the causative agent of a tick-borne disease, human monocytic ehrlichiosis. In vertebrates, E. chaffeensis exhibits tropism for monocytes /macrophages. However, no clear requirements for cell tropism have been defined in ticks. Previously, our group identified two host genes that control E. chaffeensis replication in vivo in Drosophila. We used these two genes, CG6364 and separation anxiety (san) to test the hypothesis that E. chaffeensis replicates in arthropod hemocytes. Using the UAS/GAL4 RNAi system, we generated F1 flies (RNAi flies) and confirmed ubiquitous-or tissue-specific reduction in the transcript levels of the targeted genes. When RNAi flies were screened for Ehrlichia infections, we found that when either CG6364 or san were specifically suppressed in the hemocytes or in the fat body E. chaffeensis failed to replicate or cause infection. Deletion of these genes in the eyes, wings or the salivary glands did not impact fly susceptibility or bacterial replication within these organs. Our data demonstrate that in Drosophila, E. chaffeensis replicates within the hemocytes, the insect homolog of mammalian macrophages, and in the fat body, the liver homolog of mammals. This study provides insights about replication sites of E. chaffeensis in arthropods. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Ehrlichia chaffeensis en_US
dc.subject Drosophila melanogaster en_US
dc.title Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Biology en_US
dc.description.advisor S. K. Chapes en_US
dc.subject.umi Biology (0306) en_US
dc.date.published 2012 en_US
dc.date.graduationmonth August en_US


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