Inter-relationship between sphingosine kinase and phospholipase D in signaling Arabidopsis response to abscisic acid

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Show simple item record Guo, Liang Mishra, Girish Markham, Jonathan E. Li, Maoyin Tawfall, Amanda Welti, Ruth Wang, Xuemin 2012-05-21T16:39:43Z 2012-05-21T16:39:43Z 2012-05-21
dc.description.abstract Phosphatidic acid (PA) and phytosphingosine-1-phosphate (phyto-S1P) both are lipid messengers involved in plant response to abscisic acid (ABA). Our previous data indicate that PA binds to sphingosine kinase (SPHK) and increases its phyto-S1P-producing activity. To understand the cellular and physiological functions of the PA-SPHK interaction, we isolated Arabidopsis thaliana SPHK mutants sphk1-1 and sphk2-1 and characterized them, along with phospholipase Dα1 knockout, pldα1, in plant response to ABA. Compared to wild-type (WT) plants, the SPHK mutants and pldα1 all displayed decreased sensitivity to ABA-promoted stomatal closure. Phyto-S1P promoted stomatal closure in sphk1-1 and sphk2-1, but not in pldα1, whereas PA promoted stomatal closure in sphk1-1, sphk2-1, and pldα1. The ABA activation of PLDα1 in leaves and protoplasts was attenuated in the SPHK mutants, and the ABA activation of SPHK was reduced in pldα1. In response to ABA, the accumulation of long-chain base phosphate (LCBP) was decreased in pldα1 whereas PA production was decreased in SPHK mutants, compared WT. Collectively, these results indicate that SPHK and PLDα1 act together in ABA response and that SPHK and phyto-S1P act upstream of PLDα1 and PA in mediating the ABA response. Whereas SPHKs are involved in the activation of PLDα1, the activity of PLDα1 further enhances SPHK activation via PA binding SPHKs. The data suggest that SPHK/phyto-S1P and PLDa1/PA are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis. en_US
dc.relation.uri en_US
dc.rights Published citation: Guo, L., Mishra, G., Markham, J. E., Li, M., Tawfall, A., Welti, R., & Wang, X. (2012). Connections between sphingosine kinase and phospholipase D in the abscisic acid signaling pathway in Arabidopsis. Journal of Biological Chemistry, 287(11), 8286-8296. en_US
dc.subject Abscisic acid en_US
dc.subject Phosphatidic acid en_US
dc.subject Signal transduction en_US
dc.subject Sphingosine 1-phosphate en_US
dc.subject Stress response en_US
dc.subject PA-protein interaction en_US
dc.subject Lipid signaling en_US
dc.subject Sphingosine kinase en_US
dc.title Inter-relationship between sphingosine kinase and phospholipase D in signaling Arabidopsis response to abscisic acid en_US
dc.type Article (author version) en_US 2012 en_US
dc.citation.doi doi:10.1074/jbc.M111.274274 en_US
dc.citation.epage 8296 en_US
dc.citation.issue 11 en_US
dc.citation.jtitle Journal of Biological Chemistry en_US
dc.citation.spage 8286 en_US
dc.citation.volume 287 en_US
dc.contributor.authoreid welti en_US

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