Efficacy of a vaccine and a direct-fed microbial against fecal shedding of Escherichia coli O157:H7 in a randomized pen-level field trial of commercial feedlot cattle
dc.citation.doi | 10.1016/j.vaccine.2012.05.080 | |
dc.citation.epage | 6215 | |
dc.citation.issn | 0264-410X | |
dc.citation.issue | 43 | |
dc.citation.jtitle | Vaccine | |
dc.citation.spage | 6210 | |
dc.citation.volume | 30 | |
dc.contributor.author | Cull, Charley A. | |
dc.contributor.author | Paddock, Zachary D. | |
dc.contributor.author | Nagaraja, T. G. | |
dc.contributor.author | Bello, Nora M. | |
dc.contributor.author | Babcock, Abram H. | |
dc.contributor.author | Renter, David G. | |
dc.date.accessioned | 2018-07-25T21:44:06Z | |
dc.date.available | 2018-07-25T21:44:06Z | |
dc.date.issued | 2012-09-21 | |
dc.date.published | 2012 | |
dc.description.abstract | Our primary objective was to determine the efficacy of a siderophore receptor and porin proteins-based vaccine (VAC) and a Lactobacillus acidophilus-based direct-fed microbial (DFM) against fecal shedding of Escherichia coli O157:H7 in commercial feedlot cattle fed a corn grain-based diet with 25% distiller's grains. Cattle projected to be on a finishing diet during the summer were randomly allocated into 40 study pens within ten blocks based on allocation dates. Blocks were complete; each of the four pens within a block was randomly assigned one treatment: control, VAC, DFM, or VAC+DFM. The DFM was fed (106CFU/animal/day of Lactobacillus) throughout the study periods (84–88 days) and cattle were vaccinated at enrollment and again three weeks later. Fresh fecal samples (30/pen) from pen floors were collected weekly for four consecutive weeks (study days 52–77). Two concurrent culture procedures were used to enable estimates of E. coli O157:H7 shedding prevalence and prevalence of high shedders. From 4800 total samples, 1522 (31.7%) were positive for E. coli O157:H7 and 169 (3.5%) were considered high shedders. Pen-level linear mixed models were used for data analyses. There were no significant interactions among treatments and time of sampling. However, vaccinated pens had lower (P<0.01) overall prevalence of E. coli O157:H7 (model-adjusted mean ±SEM=17.4±3.95%) and lower (P<0.01) prevalence of high shedders (0.95±0.26%) than unvaccinated pens (37.0±6.32% and 4.19±0.81%, respectively). There was no evidence of a DFM effect on either measure of E. coli O157:H7 shedding. Results indicate that a two-dose regimen of the vaccine significantly reduces fecal prevalence of E. coli O157:H7 (vaccine efficacy of 53.0%) and prevalence of E. coli O157:H7 high shedders (vaccine efficacy of 77.3%) in commercial feedlot cattle reared in the summer on a finishing diet with 25% distiller's grains. | |
dc.description.version | Article: Version of Record | |
dc.identifier.uri | http://hdl.handle.net/2097/39069 | |
dc.relation.uri | https://doi.org/10.1016/j.vaccine.2012.05.080 | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | Cattle | |
dc.subject | Feedlot | |
dc.subject | Food safety | |
dc.subject | O157:H7 | |
dc.subject | SRP | |
dc.subject | Vaccine | |
dc.title | Efficacy of a vaccine and a direct-fed microbial against fecal shedding of Escherichia coli O157:H7 in a randomized pen-level field trial of commercial feedlot cattle | |
dc.type | Text |
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