Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization
| dc.citation | Benoit, I., Zhou, M. M., Duarte, A. V., Downes, D. J., Todd, R. B., Kloezen, W., . . . de Vries, R. P. (2015). Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization. Scientific Reports, 5(1), 1-13. https://doi.org/10.1038/srep13592 | |
| dc.citation.doi | 10.1038/srep13592 | |
| dc.citation.epage | 13 | |
| dc.citation.issn | 2045-2322 | |
| dc.citation.issue | 1 | |
| dc.citation.jtitle | Scientific Reports | |
| dc.citation.spage | 1 | |
| dc.citation.volume | 5 | |
| dc.contributor.author | Benoit, I. | |
| dc.contributor.author | Zhou, M. M. | |
| dc.contributor.author | Duarte, A. V. | |
| dc.contributor.author | Downes, D. J. | |
| dc.contributor.author | Todd, Richard B. | |
| dc.contributor.author | Kloezen, W. | |
| dc.contributor.author | Post, H. | |
| dc.contributor.author | Heck, A. J. R. | |
| dc.contributor.author | Altelaar, A. F. M. | |
| dc.contributor.author | de Vries, R. P. | |
| dc.contributor.authoreid | rbtodd | |
| dc.date.accessioned | 2016-04-06T15:14:25Z | |
| dc.date.available | 2016-04-06T15:14:25Z | |
| dc.date.issued | 2015-08-28 | |
| dc.date.published | 2015 | |
| dc.description | Citation: Benoit, I., Zhou, M. M., Duarte, A. V., Downes, D. J., Todd, R. B., Kloezen, W., . . . de Vries, R. P. (2015). Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization. Scientific Reports, 5(1), 1-13. https://doi.org/10.1038/srep13592 | |
| dc.description | Degradation of plant biomass to fermentable sugars is of critical importance for the use of plant materials for biofuels. Filamentous fungi are ubiquitous organisms and major plant biomass degraders. Single colonies of some fungal species can colonize massive areas as large as five soccer stadia. During growth, the mycelium encounters heterogeneous carbon sources. Here we assessed whether substrate heterogeneity is a major determinant of spatial gene expression in colonies of Aspergillus niger. We analyzed whole-genome gene expression in five concentric zones of 5-day-old colonies utilizing sugar beet pulp as a complex carbon source. Growth, protein production and secretion occurred throughout the colony. Genes involved in carbon catabolism were expressed uniformly from the centre to the periphery whereas genes encoding plant biomass degrading enzymes and nitrate utilization were expressed differentially across the colony. A combined adaptive response of carbon-catabolism and enzyme production to locally available monosaccharides was observed. Finally, our results demonstrate that A. niger employs different enzymatic tools to adapt its metabolism as it colonizes complex environments. | |
| dc.description.version | Article: Version of Record | |
| dc.identifier.uri | http://hdl.handle.net/2097/32464 | |
| dc.relation.uri | https://doi.org/10.1038/srep13592 | |
| dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Ammonium Permease Genes | |
| dc.subject | Signal Peptides | |
| dc.subject | Microarray Data | |
| dc.subject | Proteomics | |
| dc.subject | Data | |
| dc.subject | Nidulans | |
| dc.title | Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization | |
| dc.type | Text |
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