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Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs

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dc.citation.doi10.1186/s13567-015-0180-5
dc.citation.issn0928-4249
dc.citation.jtitleVeterinary Research
dc.citation.spage9
dc.citation.volume46
dc.contributor.authorCrawford, K.
dc.contributor.authorLager, K.
dc.contributor.authorMiller, L.
dc.contributor.authorOpriessnig, T.
dc.contributor.authorGerber, P.
dc.contributor.authorHesse, Richard A.
dc.contributor.authoreidrhesse
dc.date.accessioned2016-04-04T22:45:07Z
dc.date.available2016-04-04T22:45:07Z
dc.date.issued2015-05-06
dc.date.published2015
dc.descriptionCitation: Crawford, K., Lager, K., Miller, L., Opriessnig, T., Gerber, P., & Hesse, R. (2015). Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs. Veterinary Research, 46, 9. doi:10.1186/s13567-015-0180-5
dc.descriptionClinical disease associated with porcine epidemic diarrhea virus (PEDV) infection in naive pigs is well chronicled; however, information on endemic PEDV infection is limited. To characterize chronic PEDV infection, the duration of infectious virus shedding and development of protective immunity was determined. On Day 0 (D0), a growing pig was challenged with PEDV and 13 contacts were commingled. On D7, 9 contact pigs (principal virus group (PG)), were selected, moved to a separate room and commingled with one sentinel pig (S1). This process was repeated weekly with S2, S3 and S4. The PG was PEDV-positive by PCR from D3-11, with some pigs intermittently positive to D42. Pigs S1 and S2 were PEDV-positive within 24 hours of commingling. Antibodies were detected in all PG by D21 and by 7 days post-contact in S1 and S2. Pigs S3 and S4 were PCR and antibody negative following commingling. To evaluate protective immunity, 5 naive pigs (N) and the PG were challenged (N/C, PG/C) with homologous virus on D49. All N/C pigs were PEDV PCR-positive by D52 with detection out to D62 in 3/5 N/C pigs. All PG/C pigs were PEDV PCR-negative post-challenge. By D63, all N/C seroconverted. Although PEDV RNA was demonstrated in pigs after primary infection until D42, infectious PEDV capable of horizontal transmission to naive pigs was only shed 14-16 days after infection to age-matched pigs. Homologous re-challenge 49 days post initial PEDV exposure did not result in re-infection of the pigs. This demonstrates potential for an effective PEDV vaccine.
dc.identifier.urihttp://hdl.handle.net/2097/32291
dc.relation.urihttps://doi.org/10.1186/s13567-015-0180-5
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectUnited-States
dc.subjectOutbreak
dc.subjectSwine
dc.subjectStrains
dc.subjectChina
dc.subjectVeterinary Sciences
dc.titleEvaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs
dc.typeArticle

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