Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat

dc.citation.doi10.1038/srep08092
dc.citation.issn2045-2322
dc.citation.jtitleScientific Reports
dc.citation.spage7
dc.citation.volume5
dc.contributor.authorLiu, Xuming M.
dc.contributor.authorZhang, S. Z.
dc.contributor.authorWhitworth, R. J.
dc.contributor.authorStuart, J. J.
dc.contributor.authorChen, M. S.
dc.contributor.authoreidxmliu
dc.date.accessioned2016-04-04T22:44:42Z
dc.date.available2016-04-04T22:44:42Z
dc.date.issued2015-01-28
dc.date.published2015
dc.descriptionCitation: Liu, X. M., Zhang, S. Z., Whitworth, R. J., Stuart, J. J., & Chen, M. S. (2015). Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat. Scientific Reports, 5, 7. doi:10.1038/srep08092
dc.descriptionGlutathione, gamma-glutamylcysteinylglycine, exists abundantly in nearly all organisms. Glutathione participates in various physiological processes involved in redox reactions by serving as an electron donor/acceptor. We found that the abundance of total glutathione increased up to 60% in resistant wheat plants within 72 hours following attack by the gall midge Mayetiola destructor, the Hessian fly. The increase in total glutathione abundance, however, is coupled with an unbalanced activation of glutathione metabolic pathways. The activity and transcript abundance of glutathione peroxidases, which convert reduced glutathione (GSH) to oxidized glutathione (GSSG), increased in infested resistant plants. However, the enzymatic activity and transcript abundance of glutathione reductases, which convert GSSG back to GSH, did not change. This unbalanced regulation of the glutathione oxidation/reduction cycle indicates the existence of an alternative pathway to regenerate GSH from GSSG to maintain a stable GSSG/GSH ratio. Our data suggest the possibility that GSSG is transported from cytosol to apoplast to serve as an oxidant for class III peroxidases to generate reactive oxygen species for plant defense against Hessian fly larvae. Our results provide a foundation for elucidating the molecular processes involved in glutathione-mediated plant resistance to Hessian fly and potentially other pests as well.
dc.identifier.urihttp://hdl.handle.net/2097/32271
dc.relation.urihttps://doi.org/10.1038/srep08092
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectApoplastic Oxidative Burst
dc.subjectHessian Fly Diptera
dc.subjectGene-Expression
dc.subjectHypersensitive Response
dc.subjectDisease Resistance
dc.subjectS-Transferases
dc.titleUnbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat
dc.typeArticle

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