Armet transcript knockdown in Tribolium castaneum
dc.contributor.author | Bechard, Jarrod | |
dc.date.accessioned | 2017-02-15T20:14:56Z | |
dc.date.available | 2017-02-15T20:14:56Z | |
dc.date.graduationmonth | August | en_US |
dc.date.issued | 2014-08-01 | en_US |
dc.date.published | 2014 | en_US |
dc.description.abstract | Armet has been found in mammalian systems to be a bi-functional protein that is secreted extracellularly and is also found in the endoplasmic reticulum. It has been shown to be a neurotrophic factor and also a member of the unfolded protein response. Transcript knockdown of Armet via RNA interference in late instar larvae of Tribolium castaneum produces a fatal phenotype during eclosion from pupa to adult. Initial observations of pupae cuticle indicate disorganization of cuticles in insects with the Armet transcript knocked down. Here I expand studies on the effects of dsArmet RNA injection; both in a wild type strain and a fluorescent strain of Tribolium, and discuss possible mechanisms for the fatal phenotype. | en_US |
dc.description.advisor | Gerald R. Reeck | en_US |
dc.description.degree | Master of Science | en_US |
dc.description.department | Biochemistry and Molecular Biophysics Interdepartmental Program | en_US |
dc.description.level | Masters | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/35220 | |
dc.language.iso | en_US | en_US |
dc.publisher | Kansas State University | en |
dc.subject | Tribolium castaneum | en_US |
dc.subject | RNAi | en_US |
dc.subject | Armet | en_US |
dc.subject | Cuticle | en_US |
dc.title | Armet transcript knockdown in Tribolium castaneum | en_US |
dc.type | Thesis | en_US |