Analysis of EST’s encoding pea aphid Acyrthosiphon pisum C002 & the effect of armet transcript knockdown in Tribolium castaneum
dc.contributor.author | Heerman, Matthew C. | |
dc.date.accessioned | 2014-02-03T16:08:23Z | |
dc.date.available | 2014-02-03T16:08:23Z | |
dc.date.graduationmonth | December | en_US |
dc.date.issued | 2014-02-03 | |
dc.date.published | 2012 | en_US |
dc.description.abstract | Aphids mount a remarkable salivary secretion to overcome plant host defenses. Our group has previously reported a gene unique to aphids enriched in the salivary glands of the pea aphid A. pisum, C002, which is required for successful feeding on its host plant Vicia fava. Here I present an analysis of genetic variation within the available EST data for C002 in pea aphids. From 596 total ESTs, 332 are full-length, and segregate into 8 validated haplotypes based on the criteria I set in place to access the quality of EST data. Additionally, Armet, is a putative multi-functional gene implicated as a neurotrophic factor during development, and as a part of the unfolded protein response during stress. I employ RNA interference in the model organism T. castaneum to determine the effect of transcript knockdown during development from early in-star larval stages, through pupation, and its effect on adult emergence. I report that knockdown of Armet transcript significantly hinders the ability for beetles to emerge from the pupae. | en_US |
dc.description.advisor | Gerald R. Reeck | en_US |
dc.description.degree | Master of Science | en_US |
dc.description.department | Department of Biochemistry | en_US |
dc.description.level | Masters | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/17148 | |
dc.language.iso | en_US | en_US |
dc.publisher | Kansas State University | en |
dc.subject | RNAi | en_US |
dc.subject | Armet | en_US |
dc.subject | Tribolium castaneum | en_US |
dc.subject | Neurotrophic factor | en_US |
dc.subject.umi | Biochemistry (0487) | en_US |
dc.subject.umi | Genetics (0369) | en_US |
dc.title | Analysis of EST’s encoding pea aphid Acyrthosiphon pisum C002 & the effect of armet transcript knockdown in Tribolium castaneum | en_US |
dc.type | Thesis | en_US |