Pilot study: identification of anthocyanin metabolites in the mice fed purple-fleshed sweetpotato
dc.contributor.author | Chen, Tzu-Yu | |
dc.date.accessioned | 2012-04-30T18:11:48Z | |
dc.date.available | 2012-04-30T18:11:48Z | |
dc.date.graduationmonth | May | en_US |
dc.date.issued | 2012-04-30 | |
dc.date.published | 2012 | en_US |
dc.description.abstract | Anthocyanins may prevent chronic diseases such as cancer and cardiovascular disease, however, the anthocyanin metabolites are not well elucidated. We previously selected a purple-fleshed sweet potato clone P40 that contained anthocyanins at up to 7.5 g/kg dry matter, most of which are cyanidin and peonidin derivatives. The main objective of this study is to identify anthocyanin metabolites in the mice fed 20-30% of purple sweet potato P40 (287 mg and 430 mg peonidin-3-glucoside equivalent /kg body weight) diet for 6 weeks. Plasma, liver, and feces were analyzed for anthocyanin metabolites using HPLC/MS and MALDI-TOF-MS. Fifteen hours after consumption of P40 diet, we identified 4 anthocyanin metabolites cyanidin 3,5- diglucoside; cyanidin 3-sophoroside-5-glucoside; cyanidin3-p-hydroxybenzoylsophroside-5-glucoside; and peonidin 3-p-hydroxybenzoylsophroside-5-glucoside in fecal samples. No anthocyanin metabolites were detected in plasma or liver extracts by HPLC/MS or MALDI-TOF-MS. The results indicate that anthocyanin metabolites in fecal samples might provide health benefits for colonic mucosal cells. However, the lack metabolites in both plasma and liver samples suggest a continuous intake of the anthocyanins may be required for systemic benefits due to their quick degradation and low bioavailability. | en_US |
dc.description.advisor | Weiqun Wang | en_US |
dc.description.degree | Master of Science | en_US |
dc.description.department | Department of Human Nutrition | en_US |
dc.description.level | Masters | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/13764 | |
dc.language.iso | en_US | en_US |
dc.publisher | Kansas State University | en |
dc.subject | Anthocyanin | en_US |
dc.subject | Purple sweet potato | en_US |
dc.subject | Bioavailability | en_US |
dc.subject.umi | Nutrition (0570) | en_US |
dc.title | Pilot study: identification of anthocyanin metabolites in the mice fed purple-fleshed sweetpotato | en_US |
dc.title.alternative | Pilot study: identification of anthocyanin metabolites in the mice fed purple-fleshed sweet potato | en_US |
dc.type | Thesis | en_US |