Beta-cyclodextrin complexing to reduce antibiotic residue in milk

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dc.contributor.author Banala, S.
dc.contributor.author Brouk, Michael J.
dc.contributor.author Simmons, S.R.
dc.contributor.author Schmidt, Karen A.
dc.date.accessioned 2010-12-01T22:17:52Z
dc.date.available 2010-12-01T22:17:52Z
dc.date.issued 2010-12-01
dc.identifier.uri http://hdl.handle.net/2097/6720
dc.description.abstract Various percentages (1.2% to 13%) of β- cyclodextrin (β–CD) were added to water or pasteurized whole milk to study β-CD crystallization patterns. Influential factors such as crystallization time (4 to 12 h), crystallization temperature (45º vs. 72ºF), and centrifugation speed (25 to 3000 × g) were investigated. Optimized crystallization conditions were verified in antibiotic-tainted raw milk samples via the enzyme-linked receptor-binding assay and by solids partitioning. In water, β-CD precipitate increased significantly as β-CD concentration and crystallization time increased, but was independent of the centrifugation speed. In pasteurized whole milk, precipitate increased as β-CD concentration, crystallization time, and centrifugation speed increased. The best β-CD precipitation conditions in milk were designated as: 9.1% β-CD concentration (w/w), 4 hour crystallization time, 45ºF, and centrifugation at 25 × g for 10 minutes. Eleven cows were treated with cephapirin sodium or cephapirin benzathine. Milk was obtained 12 h later and treated with β-CD. As β- CD concentrations increased, precipitates increased, and the corresponding supernatants showed reduced concentrations of antibiotics when tested by enzyme-linked receptorbinding assay. Results indicated that β-CD may have the potential to reduce the residues of cephapirin sodium or cephapirin benzathine in antibiotic-tainted raw milk. en_US
dc.publisher Kansas State University. Agricultural Experiment Station and Cooperative Extension Service en_US
dc.relation.isPartOf Dairy Day, 2004 en_US
dc.relation.isPartOf Kansas Agricultural Experiment Station contribution; no. 05-112-S en_US
dc.relation.isPartOf Report of progress (Kansas State University. Agricultural Experiment Station and Cooperative Extension Service); 941 en_US
dc.subject Dairy en_US
dc.subject β-cyclodextrin en_US
dc.subject Cephapirin en_US
dc.subject Sodium en_US
dc.subject Cephapirin benzathine en_US
dc.subject Milk en_US
dc.title Beta-cyclodextrin complexing to reduce antibiotic residue in milk en_US
dc.type Conference paper en_US
dc.date.published 2004 en_US
dc.citation.epage 15 en_US
dc.citation.spage 10 en_US
dc.contributor.authoreid mbrouk en_US
dc.contributor.authoreid schmidt en_US

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