Use of Oxyrase® enzyme to enhance recovery of Escherichia coli O157:H7 from culture media and ground beef

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dc.contributor.author Thippareddi, H.
dc.contributor.author Kone, K.
dc.contributor.author Phebus, Randall K.
dc.contributor.author Fung, Daniel Y. C.
dc.contributor.author Kastner, Curtis L.
dc.date.accessioned 2010-10-05T17:53:41Z
dc.date.available 2010-10-05T17:53:41Z
dc.date.issued 2010-10-05T17:53:41Z
dc.identifier.uri http://hdl.handle.net/2097/6256
dc.description.abstract Escherichia coli O157:H7 is a bacterium that has caused great concern in the meat and food industry during the last few years because of several, well-publicized, disease outbreaks, including the incident at the Jack-in- the-Box fast food chain in Seattle, Washington. The organism can cause severe sickness and even death in certain population groups. To better assure meat safety, federal meat inspection is focusing on developing rapid methods to detect this disease agent and others. Oxyrase is a commercially available enzyme that can accelerate the growth of some bacteria. Current techniques for isolation and culturing of E. coli O157:H7 from foods require an enrichment period of 18 to 24 hours, thus limiting their usefulness for perishable foods that are marketed quickly. Our investigation found that Oxyrase shortened required enrichment periods in broth culture only. The enzyme was less effective in sterilized ground beef. en_US
dc.publisher Kansas State University. Agricultural Experiment Station and Cooperative Extension Service en_US
dc.relation.isPartOf Cattlemen’s Day, 1994 en_US
dc.relation.isPartOf Kansas Agricultural Experiment Station contribution; no. 94-373-S en_US
dc.relation.isPartOf Report of progress (Kansas State University. Agricultural Experiment Station and Cooperative Extension Service); 704 en_US
dc.subject Beef en_US
dc.subject Escherichia coli O157:H7 en_US
dc.subject Oxyrase en_US
dc.subject Ground beef en_US
dc.subject Rapid methodology en_US
dc.subject Meat safety en_US
dc.title Use of Oxyrase® enzyme to enhance recovery of Escherichia coli O157:H7 from culture media and ground beef en_US
dc.type Conference paper en_US
dc.date.published 1994 en_US
dc.citation.epage 133 en_US
dc.citation.spage 131 en_US
dc.description.conference Cattlemen's Day, 1994, Kansas State University, Manhattan, KS, March 4, 1994 en_US
dc.contributor.authoreid phebus en_US
dc.contributor.authoreid dfung en_US
dc.contributor.authoreid ckastner en_US

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