Abstract:
Ruminal degradation of organic matter
and protein in alfalfa and prairie hay were
evaluated in vivo, using cannulated cows, and
in vitro, using a continuous-culture fermenter
to simulate ruminal fermentation. Estimates
of organic matter degradability, microbial N
flow per unit feed N input, and efficiency of
microbial growth were not different (P>.10)
between the in vivo and in vitro systems.
However, for both forages, estimates of
nitrogen degradability were greater with the
in vitro system. Despite the differences
between in vivo and in vitro techniques for
some variables, continuous-culture
fermentation will allow us to compare the
effects of dietary treatments on forage
digestion and will aid in the formulation of
supplements to meet specific nutrient
requirements for cattle consuming forage-based
diets.