Abstract:
Six ruminally cannulated Holstein steers
(550 lb) implanted with Revalor-S were infused
abomasally with water or a mixture of six amino
acids in a crossover experiment (two 14-day
periods) to evaluate effects on nitrogen balance.
The mixture was comprised of amino acids that
potentially may be limiting in lightweight steers,
namely (g/day): lysine (5.3), methionine (3.3),
threonine (3.2), tryptophan (1 .0), histidine (2.1),
and arginine (5.5). Steers were fed at levels just
below ad libitum intake. The diet contained
86% rolled corn, 10% prairie hay, 3% mineral
and vitamin premixes, and 1% urea (as-fed).
Amino acid infusion increased nitrogen
retention by 17.9% over the control, from 27.9
g N/day to 32.9 g N/day. This indicates that
implanted steers fed a high concentrate diet are
able to respond to amino acid supplementation,
suggesting that at least one of the infused amino
acids was limiting in the basal corn-urea diet.