Effect of melengestrol acetate (MGA) on cultured bovine muscle satellite cell proliferation and differentiation

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dc.contributor.author Sissom, E.K.
dc.contributor.author Kayser, J.P.
dc.contributor.author Waylan, A.T.
dc.contributor.author Dunn, J.D.
dc.contributor.author Johnson, B.J.
dc.date.accessioned 2010-08-04T17:41:40Z
dc.date.available 2010-08-04T17:41:40Z
dc.date.issued 2010-08-04T17:41:40Z
dc.identifier.uri http://hdl.handle.net/2097/4479
dc.description.abstract Melengestrol acetate (MGA) increases growth rate and inhibits estrus in feedlot heifers. Little is known of MGA’s effect on skeletal muscle growth and differentiation. The purpose of this trial was to investigate the potential direct effects of MGA on cultured bovine muscle satellite cell proliferation and differentiation. Satellite cells isolated from yearling cattle were used to assess the effect of MGA in a dose titration (0, 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, and 100 μM) study on [3H]-thymidine incorporation. Likewise, satellite cell cultures were allowed to differentiate, and nuclei were stained at 168 hours to determine the effect of MGA (10 nM and 100 μM) addition during the first 48 hours on extent of differentiation and absolute myotube nuclei number. MGA addition resulted in a dose-dependent decrease (P<0.05) in DNA synthesis as measured by [3H]-thymidine incorporation. MGA addition (10 nM) did not significantly alter the extent of differentiation or myotube nuclei number at 168 hours in culture even though this concentration reduced DNA synthesis. However, 100 μM MGA addition significantly (P<0.05) reduced both fusion percentage and myotube nuclei number as compared to control cultures. These data suggest MGA addition at concentration between 10 nM and 100 μM affected bovine muscle cell proliferation and differentiation. A better understanding of these effects will increase our knowledge of bovine muscle growth and development. en_US
dc.publisher Kansas State University. Agricultural Experiment Station and Cooperative Extension Service en_US
dc.relation.isPartOf Cattlemen's Day, 2003 en_US
dc.relation.isPartOf Kansas Agricultural Experiment Station contribution; no. 03-272-S en_US
dc.relation.isPartOf Report of progress (Kansas State University. Agricultural Experiment Station and Cooperative Extension Service); 908 en_US
dc.subject Beef en_US
dc.subject Melengestrol acetate (MGA) en_US
dc.subject Satellite cell en_US
dc.subject Proliferation en_US
dc.subject Differentiation en_US
dc.title Effect of melengestrol acetate (MGA) on cultured bovine muscle satellite cell proliferation and differentiation en_US
dc.type Conference paper en_US
dc.date.published 2003 en_US
dc.citation.epage 210 en_US
dc.citation.spage 207 en_US
dc.description.conference Cattlemen's Day, 2003, Kansas State University, Manhattan, KS, March 7, 2003 en_US

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