Genomic targeting and mapping of a gametocidal gene in wheat


Show simple item record See, Deven R. 2007-11-26T17:52:03Z 2007-11-26T17:52:03Z 2007-11-26T17:52:03Z December 2007 en
dc.description.abstract Segregation distortion describes the transmission of an allele or alleles of a heterozygous locus at a higher frequency than expected in a Mendelian ratio. From the organism's view, segregation distortion is the preferential retention of chromosomal blocks carrying genes beneficial to its fitness and reproductive viability. In wheat the best studied segregation distortes are those introduced from Aegilops species; these selfish genetic elements are named gametocidal (Gc) genes and the chromosomes carrying them are called Gc chromosomes. This genetic mechanism causes chromosome breakage in gametophytes lacking the Gc carrier chromosome, thus favoring its own retention in the genome. While the mode of action of the Gc genes is not yet known, they have been used extensively in wheat genetics for the development of deletion stocks, a key resource for elucidating the structure of physical regions containing important genes. The objective of this study was to develop the tools necessary to map the Gc2 gene derived from Ae. sharonensis and perform map-based cloning. Extensive physical and genetic mapping located the gametocidal gene on the distal 1% of the 4BL arm present in the T4BS[dot in middle of line]4BL-4S[sh superscript]#1L translocation chromosome. Comparative genomics using rice provided markers distal and proximal to the Gc2 locus; however, synteny broke down at the locus. The characterization of this chromosomal region has provided insight into its recombination frequency, synteny and composition; however, the dynamic architecture of the end of the chromosome has made comparative mapping of this region difficult. en
dc.description.sponsorship United States Department of Agriculture National Needs Fellowship en
dc.language.iso en_US en
dc.publisher Kansas State University en
dc.subject Wheat en
dc.subject Gametocidal gene en
dc.subject Aegilops sharonensis en
dc.subject Triticum aestivum en
dc.title Genomic targeting and mapping of a gametocidal gene in wheat en
dc.type Dissertation en Doctor of Philosophy en
dc.description.level Doctoral en
dc.description.department Department of Plant Pathology en
dc.description.advisor Bikram S. Gill en
dc.subject.umi Biology, Genetics (0369) en 2007 en December en

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