The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae

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dc.contributor.author Zheng, H. K.
dc.contributor.author Chen, S. M.
dc.contributor.author Chen, X. F.
dc.contributor.author Liu, S. Y.
dc.contributor.author Dang, X.
dc.contributor.author Yang, C. D.
dc.contributor.author Giraldo, M. C.
dc.contributor.author Oliveira-Garcia, E.
dc.contributor.author Zhou, J.
dc.contributor.author Wang, Z. H.
dc.contributor.author Valent, Barbara
dc.date.accessioned 2017-11-30T21:53:41Z
dc.date.available 2017-11-30T21:53:41Z
dc.identifier.uri http://hdl.handle.net/2097/38412
dc.description Citation: Zheng, H. K., Chen, S. M., Chen, X. F., Liu, S. Y., Dang, X., Yang, C. D., . . . Valent, B. (2016). The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae. Frontiers in Plant Science, 7, 14. doi:10.3389/fpls.2016.01458
dc.description.abstract The Rab GTPase proteins play important roles in the membrane trafficking, and consequently protein secretion and development of eukaryotic organisms. However, little is known about the function of Rab GTPases in Magnaporthe oryzae. To further explore the function of Rab GTPases, we deleted the ortholog of the yeast Sec4p protein in M. oryzae, namely MoSEC4. The Delta Mosec4 mutant is defective in polarized growth and conidiation, and it displays decreased appressorium turgor pressure and attenuated pathogenicity. Notably, the biotrophic invasive hyphae produced in rice cells are more bulbous and compressed in the Delta Mosec4 mutant. Further studies showed that deletion of the MoSEC4 gene resulted in decreased secretion of extracellular enzymes and mislocalization of the cytoplasmic effector PWL2-mCherry-NLS. In accordance with a role in secretion, the GFP-MoSec4 fusion protein mainly accumulates at tips of growing vegetative hyphae. Our results suggest that the MoSec4 protein plays important roles in the secretion of extracellular proteins and consequently hyphal development and pathogenicity in the rice blast fungus.
dc.relation.uri https://doi.org/10.3389/fpls.2016.01458
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Rab Gtpase
dc.subject Exocyst Complex
dc.subject Mosec4
dc.subject Effector Secretion
dc.subject Pyricularia
dc.subject Oryzae
dc.title The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion in Magnaporthe oryzae
dc.type Article
dc.date.published 2016
dc.citation.doi 10.3389/fpls.2016.01458
dc.citation.issn 1664-462X
dc.citation.jtitle Frontiers in Plant Science
dc.citation.spage 14
dc.citation.volume 7
dc.contributor.authoreid bvalent
dc.contributor.kstate Valent, Barbara


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