Ecological genomics: making the leap from model systems in the lab to native populations in the field.


Show simple item record Travers, Steven E. Smith, Melinda D. Bai, Jianfa Hulbert, Scot H. Leach, Jan E. Schnable, Patrick S. Knapp, Alan K. Milliken, George A. Fay, Philip A. Saleh, Amgad Garrett, Karen A. 2010-01-11T22:33:47Z 2010-01-11T22:33:47Z 2010-01-11T22:33:47Z
dc.description.abstract Recent reviews have emphasized the need to incorporate genomics into ecological field studies to further understand how species respond to changing environmental conditions. Genomic tools, such as cDNA (complementary DNA) microarrays, allow for the simultaneous analysis of gene expression of thousands of genes from all or part of an organism’s genome (the transcription profile), thereby revealing the genetic mechanisms that underlie species’ responses to environmental change. However, despite their potential, two major limitations have hindered the incorporation of microarrays and other genomic tools into field studies: (1) the limited availability of microarrays for ecologically relevant, non-model species and limited financial resources for developing new microarrays; and (2) concern that high sensitivity of gene expression to even subtle alterations in environmental conditions will hinder detection of relevant changes in field measures of transcription profiles. Here, we show that with cross-species hybridizations of microarrays developed for a closely related model organism, an appropriate experimental design, and sufficient replication, transcriptional profiling can successfully be incorporated into field studies. In this way, relevant changes in gene expression with changing environmental conditions can be detected. en_US
dc.subject Gene expression en_US
dc.subject Arabidopsis thaliana en_US
dc.subject Microarrays en_US
dc.subject Grasses en_US
dc.subject Stress en_US
dc.subject Maize en_US
dc.title Ecological genomics: making the leap from model systems in the lab to native populations in the field. en_US
dc.type Article (publisher version) en_US 2007 en_US
dc.citation.epage 24 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Frontiers in Ecology and the Environment en_US
dc.citation.spage 19 en_US
dc.citation.volume 5 en_US
dc.contributor.authoreid kgarrett en_US

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