Comparative studies on the effects of water, ethanol and water/ethanol mixtures on chemical partitioning into porcine stratum corneum and silastic membrane

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dc.contributor.author van der Merwe, Deon
dc.contributor.author Riviere, Jim E.
dc.date.accessioned 2009-10-20T19:47:03Z
dc.date.available 2009-10-20T19:47:03Z
dc.date.issued 2009-10-20T19:47:03Z
dc.identifier.uri http://hdl.handle.net/2097/1907
dc.description.abstract The effects of water and ethanol vehicles on stratum corneum and silastic membrane partitioning of 11 industrial and agricultural compounds were studied to aid in characterizing and assessing risk from skin exposure. Zero percent, 50% and 100% aqueous ethanol solutions were used as solvents for 14C labeled phenol, 4-nitrophenol, pentachlorophenol, dimethyl parathion, parathion, chloropyrifos, fenthion, triazine, atrazine, simazine and propazine. Compound partitioning between the solvents and porcine stratum corneum/silastic membrane were estimated. Stratum corneum was exposed to aqueous ethanol ranging from 0% to 100% v/v ethanol in 20% increments and Fourier transform infrared spectroscopy (FT-IR) was used to obtain an index of lipid disorder. Gravimetry and FT-IR were used to demonstrate lipid extraction in aqueous ethanol solutions. Partitioning patterns in silastic membranes resembled those in stratum corneum and were correlated with octanol/water partitioning. Partitioning was highest in water and was higher from 50% ethanol than from 100% ethanol, except for parathion, 4-nitrophenol, atrazine and propazine. Correlation existed between molecular weight and partitioning in water, but not in ethanol and ethanol/water mixtures. Lipid order, as reflected in FTIR spectra, was not altered. These studies suggest that stratum corneum partitioning of the compounds tested is primarily determined by relative compound solubility between the stratum corneum lipids and the donor solvent. Linear relationships existed between octanol/water partitioning and stratum corneum partitioning. Partitioning was also correlated with molecular weight in water solvent systems, but not in ethanol and ethanol/water mixtures. Ethanol and ethanol/water mixtures altered the stratum corneum through lipid extraction, rather than through disruption of lipid order. en_US
dc.relation.uri http://www.sciencedirect.com/science/journal/08872333 en_US
dc.subject Dermal absorption en_US
dc.subject Partitioning en_US
dc.subject Chemical mixtures en_US
dc.title Comparative studies on the effects of water, ethanol and water/ethanol mixtures on chemical partitioning into porcine stratum corneum and silastic membrane en_US
dc.type Article (author version) en_US
dc.date.published 2005 en_US
dc.citation.epage 77 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Toxicology In Vitro en_US
dc.citation.spage 69 en_US
dc.citation.volume 19 en_US
dc.contributor.authoreid dmerwe en_US

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