L-carnitine supplementation to gestating gilts alters the IGF axis in porcine embyronic myoblasts

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dc.contributor.author Brown, K.R.
dc.contributor.author Johnson, B.J.
dc.contributor.author Woodworth, J.C.
dc.contributor.author Goodband, Robert D.
dc.contributor.author Tokach, Michael D.
dc.contributor.author Nelssen, Jim L.
dc.date.accessioned 2009-10-20T15:54:14Z
dc.date.available 2009-10-20T15:54:14Z
dc.date.issued 2009-10-20T15:54:14Z
dc.identifier.uri http://hdl.handle.net/2097/1869
dc.description.abstract We determined the effects of supplemental L-carnitine on the insulin-like growth factor (IGF) system in porcine embryonic myoblasts (PEM) from gilts. Forty gilts (BW = 303.6 lb) were allotted to 1 of 4 treatments that were arranged in a 2 × 2 factorial, with main effects of L-carnitine (0 or 50 ppm) and day of gestation (55 or 70). All gilts were fed 3.86 lb/day and a top-dress containing either 0 or 50 ppm of L-carnitine, starting on the first day of breeding and continuing through the allotted gestation length. At d 55 or 70 of gestation, fetuses were removed for isolation of PEM from the hind-limb muscles. Real-time quantitative PCR was used to determine growth factor messenger RNA (mRNA) expression in cultured PEM at 72-, 96-, 120-, and 144-h after plating. Flow cytometry was used to analyze percentage of myogenic cells with a myoblast/myotube specific monoclonal antibody 5.1H11, and for determination of cell cycle stage. There was no treatment differences (P>0.10) for the expression of IGF-I, IGF-II, or IGFBP-5 mRNA levels. But PEM isolated from fetuses collected from gilts fed diets with L-carnitine had lower (P = 0.08) IGFBP-3 mRNA levels, compared with levels in the controls. Myoblasts isolated from fetuses from gilts fed diets with added Lcarnitine had greater (P = 0.09; 8.8%) 5.1H11 monoclonal antibody attachment, compared with the controls, after 72 hours in culture (91.8% vs. 87.4%). Although not significant (P = 0.31), the total number of PEM in the S phase of the cell cycle was 4.7% greater in PEM collected from fetuses obtained from gilts fed diets with L-carnitine, compared with numbers from the control-fed gilts (37.5% vs. 34.2%). These data suggest that L-carnitine influences the IGF system, stage of the cell cycle, and recognition of muscle development, resulting in enhanced proliferation and delayed differentiation of PEM. en_US
dc.publisher Kansas State University. Agricultural Experiment Station and Cooperative Extension Service en_US
dc.relation.isPartOf Kansas Agricultural Experiment Station contribution; no. 08-83-S en_US
dc.relation.isPartOf Swine day, 2006 en_US
dc.relation.isPartOf Report of progress (Kansas State University. Agricultural Experiment Station and Cooperative Extension Service); 966 en_US
dc.subject Insulin-like growth factor en_US
dc.subject Insulin-like growth factor binding proteins en_US
dc.subject L-carnitine en_US
dc.subject Messenger RNA en_US
dc.subject Myoblasts en_US
dc.subject Pigs en_US
dc.subject Swine en_US
dc.title L-carnitine supplementation to gestating gilts alters the IGF axis in porcine embyronic myoblasts en_US
dc.type Conference paper en_US
dc.date.published 2006 en_US
dc.citation.epage 12 en_US
dc.citation.spage 7 en_US
dc.contributor.authoreid goodband en_US
dc.contributor.authoreid mtokach en_US
dc.contributor.authoreid jnelssen en_US

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