Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry

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dc.contributor.author Li, Maoyin
dc.contributor.author Baughman, Ethan
dc.contributor.author Roth, Mary R.
dc.contributor.author Han, Xianlin
dc.contributor.author Welti, Ruth
dc.contributor.author Wang, Xuemin
dc.date.accessioned 2014-04-17T19:36:17Z
dc.date.available 2014-04-17T19:36:17Z
dc.date.issued 2014-04-17
dc.identifier.uri http://hdl.handle.net/2097/17324
dc.description.abstract Plant triacylglycerols (TAGs), or vegetable oils, provide approximately 25% of dietary calories to humans and are becoming an increasingly important source of renewable bioenergy and industrial feedstocks. TAGs are assembled, by multiple enzymes in the endoplasmic reticulum, from building blocks that include an invariable glycerol backbone and variable fatty acyl chains. It remains a challenge to elucidate the mechanism of synthesis of hundreds of different TAG species in planta. One reason is the lack of an efficient analytical approach quantifying individual molecular species. Here we report a rapid and quantitative TAG profiling approach for Arabidopsis seeds based on electrospray ionization tandem mass spectrometry with direct infusion and multiple neutral loss scans. The levels of 93 TAG molecular species, identified by their acyl components, were determined in Arabidopsis seeds. Quantitative TAG pattern analyses revealed that the TAG assembly machinery preferentially produces TAGs with one elongated fatty acid. The importance of the selectivity in oil synthesis was consistent with an observation that an Arabidopsis mutant overexpressing a patatin-like phospholipase had enhanced seed oil content with elongated fatty acids. This quantitative TAG profiling approach should facilitate investigations aimed at understanding the biochemical mechanisms of TAG metabolism in plants. en_US
dc.language.iso en_US en_US
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1111/tpj.12365/full en_US
dc.rights This is the peer reviewed version of the following article: Li, M., Baughman, E., Roth, M. R., Han, X., Welti, R. and Wang, X. (2014). Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry. The Plant Journal, 77(1), 160–172., which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/tpj.12365/full en_US
dc.subject Arabidopsis thaliana en_US
dc.subject Mass spectrometry en_US
dc.subject Lipidomics en_US
dc.subject Seed oil en_US
dc.subject Triacylglycerol profiling en_US
dc.title Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry en_US
dc.type Article (author version) en_US
dc.date.published 2014 en_US
dc.citation.doi doi:10.1111/tpj.12365 en_US
dc.citation.epage 172 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle Plant Journal en_US
dc.citation.spage 160 en_US
dc.citation.volume 77 en_US
dc.contributor.authoreid mrroth en_US
dc.contributor.authoreid welti en_US


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