Molecular studies of the salivary glands of the pea aphid, Acyrthosiphon pisum (Harris)

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dc.contributor.author Mutti, Navdeep S.
dc.date.accessioned 2006-05-08T16:53:38Z
dc.date.available 2006-05-08T16:53:38Z
dc.date.issued 2006-05-08T16:53:38Z
dc.date.submitted May 2006 en
dc.identifier.uri http://hdl.handle.net/2097/154
dc.description.abstract Salivary secretions are a key component of aphid-plant interactions. Aphids’ salivary proteins interact with plant tissues, gaining access to phloem sap and eliciting responses which may benefit the insect. In an effort to isolate and identify key components in salivary secretions, we created a salivary gland cDNA library. Several thousand randomly selected cDNA clones were sequenced. We grouped these sequences into 1769 sets of essentially identical sequences, or clusters. About 22% of the clusters matched clearly to (non-aphid) proteins of known function. Among our cDNAs, we have identified putative oxido-reductases and hydrolases that may be involved in the insect's attack on plant tissue. C002 represents an abundant transcript among the genes expressed in the salivary glands. This cDNA encodes a novel protein that fails to match to proteins outside of aphids and is of unknown function. In situ hybridization and immunohistochemistry localized C002 in the same sub-set of cells within the principal salivary gland. C002 protein was detected in fava beans that were exposed to aphids, verifying that C002 protein is a secreted protein. Injection of siC002-RNA caused depletion of C002 transcript levels dramatically over a 3 day period after injection. With a lag of 1 – 2 days, the siC002-RNA injected insects died, on average 8 days before the death of control insects injected with siRNA for green fluorescent protein. It appears, therefore, that siRNA injections of adults will be a useful tool in studying the roles of individual transcripts in aphid salivary glands. en
dc.format.extent 1571786 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher Kansas State University en
dc.subject Salivary Ggands en
dc.subject Pea aphid en
dc.subject RNAi en
dc.title Molecular studies of the salivary glands of the pea aphid, Acyrthosiphon pisum (Harris) en
dc.type Dissertation en
dc.description.degree Doctor of Philosophy en
dc.description.level Doctoral en
dc.description.department Department of Entomology en
dc.description.advisor Gerald R. Reeck en
dc.description.advisor John C. Reese en
dc.subject.umi Biology, Entomology (0353) en
dc.subject.umi Biology, Molecular (0307) en
dc.date.published 2006 en
dc.date.graduationmonth May en

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