hMSC production in disposable bioreactors with regards to GMP and PAT

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dc.contributor.author Cierpka, Katharina
dc.contributor.author Elseberg, Christiane L.
dc.contributor.author Niss, Knut
dc.contributor.author Kassem, Moustapha
dc.contributor.author Salzig, Denise
dc.contributor.author Czermak, Peter
dc.date.accessioned 2013-04-10T15:04:39Z
dc.date.available 2013-04-10T15:04:39Z
dc.date.issued 2013-04-10
dc.identifier.uri http://hdl.handle.net/2097/15485
dc.description.abstract Reactor concepts for human mesenchymal stem cell (hMSC) production are introduced. Thereby, special interest is laid on the realization of these concepts as disposables fulfilling the GMP and PAT requirements. The specialty of the hMSC production process is the cell itself being the product. This results in completely different process requirements compared to e.g. protein production in mammalian cells. Thus, great attention has to be given to the shear sensitivity of the cells. The cultivation and the harvest of the cells have to be very gentle to neither influence cell viability nor cell differentiability. Further, the production process should not cause any undesirable cell changes. For hMSC production, cell harvest is the main challenging process step. The reactor concepts should be suitable for hMSC production for clinical trials as ATMPs. Therefore, disposable systems are especially applicable. The review describes more detailed bone marrow-derived hMSC production in a disposable stirred tank reactor as promising reactor concept. en_US
dc.language.iso en_US en_US
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1002/cite.201200151/abstract en_US
dc.subject Disposable reactor en_US
dc.subject Fixed-bed reactor en_US
dc.subject hMSC en_US
dc.subject Stem cell production en_US
dc.subject Stirred-tank reactor en_US
dc.title hMSC production in disposable bioreactors with regards to GMP and PAT en_US
dc.type Article (publisher version) en_US
dc.date.published 2013 en_US
dc.citation.doi doi:10.1002/cite.201200151 en_US
dc.citation.epage 75 en_US
dc.citation.issue 1-2 en_US
dc.citation.jtitle Chemie Ingenieur Technik en_US
dc.citation.spage 67 en_US
dc.citation.volume 85 en_US
dc.contributor.authoreid pczermak en_US


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