Characterization of cDNAs encoding serine proteases and their transcriptional responses to Cry1Ab protoxin in the gut of Ostrinia nubilalis larvae

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Show simple item record Yao, Jianxiu Buschman, Lawrent L. Oppert, Brenda Khajuria, Chitvan Zhu, Kun Yan 2012-11-13T16:30:58Z 2012-11-13T16:30:58Z 2012-11-13
dc.description.abstract Serine proteases, such as trypsin and chymotrypsin, are the primary digestive enzymes in lepidopteran larvae, and are also involved in Bacillus thuringiensis (Bt) protoxin activation and protoxin/toxin degradation. We isolated and sequenced 34 cDNAs putatively encoding trypsins, chymotrypsins and their homologs from the European corn borer (Ostrinia nubilalis) larval gut. Our analyses of the cDNA-deduced amino acid sequences indicated that 12 were putative trypsins, 12 were putative chymotrypsins, and the remaining 10 were trypsin and chymotrypsin homologs that lack one or more conserved residues of typical trypsins and chymotrypsins. Reverse transcription PCR analysis indicated that all genes were highly expressed in gut tissues, but one group of phylogenetically-related trypsin genes, OnTry-G2, was highly expressed in larval foregut and midgut, whereas another group, OnTry-G3, was highly expressed in the midgut and hindgut. Real-time quantitative PCR analysis indicated that several trypsin genes (OnTry5 and OnTry6) were significantly up-regulated in the gut of third-instar larvae after feeding on Cry1Ab protoxin from 2 to 24 h, whereas one trypsin (OnTry2) was down-regulated at all time points. Four chymotrypsin and chymotrypsin homolog genes (OnCTP2, OnCTP5, OnCTP12 and OnCTP13) were upregulated at least 2-fold in the gut of the larvae after feeding on Cry1Ab protoxin for 24 h. Our data represent the first indepth study of gut transcripts encoding expanded families of protease genes in O. nubilalis larvae and demonstrate differential expression of protease genes that may be related to Cry1Ab intoxication and/or resistance. en_US
dc.language.iso en_US en_US
dc.relation.uri|utmccn=%28direct%29|utmcmd=%28none%29&__utmv=-&__utmk=2351479 en_US
dc.subject Ostrinia nubilalis en_US
dc.subject Serine proteases en_US
dc.subject cDNA en_US
dc.subject Cry1Ab protoxin en_US
dc.title Characterization of cDNAs encoding serine proteases and their transcriptional responses to Cry1Ab protoxin in the gut of Ostrinia nubilalis larvae en_US
dc.type Article (publisher version) en_US 2012 en_US
dc.citation.doi doi:10.1371/journal.pone.0044090 en_US
dc.citation.epage e44090 en_US
dc.citation.issue 8 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e44090 en_US
dc.citation.volume 7 en_US
dc.contributor.authoreid lbuschma en_US
dc.contributor.authoreid jianxiuy en_US
dc.contributor.authoreid kzhu en_US

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