Transmission electron microscopy reveals distinct macrophage- and tick cell-specific morphological stages of Ehrlichia chaffeensis

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dc.contributor.author Dedonder, Sarah E.
dc.contributor.author Cheng, Chuanmin Cheng
dc.contributor.author Willard, Lloyd H.
dc.contributor.author Boyle, Daniel L.
dc.contributor.author Ganta, Roman R.
dc.date.accessioned 2012-11-08T20:49:18Z
dc.date.available 2012-11-08T20:49:18Z
dc.date.issued 2012-11-08
dc.identifier.uri http://hdl.handle.net/2097/14928
dc.description.abstract Background: Ehrlichia chaffeensis is an emerging tick-borne rickettsial pathogen responsible for human monocytic ehrlichiosis. Despite the induction of an active host immune response, the pathogen has evolved to persist in its vertebrate and tick hosts. Understanding how the organism progresses in tick and vertebrate host cells is critical in identifying effective strategies to block the pathogen transmission. Our recent molecular and proteomic studies revealed differences in numerous expressed proteins of the organism during its growth in different host environments. Methodology/Principal Findings: Transmission electron microscopy analysis was performed to assess morphological changes in the bacterium within macrophages and tick cells. The stages of pathogen progression observed included the attachment of the organism to the host cells, its engulfment and replication within a morulae by binary fission and release of the organisms from infected host cells by complete host cell lysis or by exocytosis. E. chaffeensis grown in tick cells was highly pleomorphic and appears to replicate by both binary fission and filamentous type cell divisions. The presence of Ehrlichia-like inclusions was also observed within the nucleus of both macrophages and tick cells. This observation was confirmed by confocal microscopy and immunoblot analysis. Conclusions/Significance: Morphological differences in the pathogen’s progression, replication, and processing within macrophages and tick cells provide further evidence that E. chaffeensis employs unique host-cell specific strategies in support of adaptation to vertebrate and tick cell environments. en_US
dc.language.iso en_US en_US
dc.relation.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036749 en_US
dc.subject Ehrlichia chaffeensis en_US
dc.subject Transmission electron microscopy en_US
dc.subject Tick cells en_US
dc.subject Macrophage en_US
dc.subject Human monocytic ehrlichiosis en_US
dc.subject Rickettsial pathogens en_US
dc.title Transmission electron microscopy reveals distinct macrophage- and tick cell-specific morphological stages of Ehrlichia chaffeensis en_US
dc.type Article (publisher version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:10.1371/journal.pone.0036749 en_US
dc.citation.epage e36749 en_US
dc.citation.issue 5 en_US
dc.citation.jtitle PLoS One en_US
dc.citation.spage e36749 en_US
dc.citation.volume 7 en_US
dc.contributor.authoreid dboyle en_US
dc.contributor.authoreid sdedonder en_US
dc.contributor.authoreid ccheng en_US
dc.contributor.authoreid lwillard en_US
dc.contributor.authoreid rganta en_US

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