Polymorphisms in salivary-gland transcripts of Russian wheat aphid biotypes 1 and 2

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dc.contributor.author Cui, Feng
dc.contributor.author Smith, C. Michael
dc.contributor.author Reese, John C.
dc.contributor.author Edwards, Owain
dc.contributor.author Reeck, Gerald R.
dc.date.accessioned 2012-10-01T13:46:43Z
dc.date.available 2012-10-01T13:46:43Z
dc.date.issued 2012-10-01
dc.identifier.uri http://hdl.handle.net/2097/14765
dc.description.abstract The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko) (Homoptera: Aphididae), is a major pest of small grains. As with plant-feeding aphids in general, the interaction between RWA and host plants is governed, on the insect side, by proteins and enzymes in saliva. In this work, we examined sequence variations in transcripts encoding proteins and enzymes of RWA salivary glands. We conducted reverse transcription – polymerase chain reaction in RWA biotypes 1 and 2 using primers derived from pea aphid orthologs, and cloned regions of 17 putative salivary gland transcripts. For four of the transcripts, we observed no difference in sequences between the two biotypes. For the other 13 transcripts, for example, the transcripts encoding sucrase, trehalase and protein C002, large amount of variations, both within each biotype and between the two biotypes, were observed. Usually the two biotypes shared only one variant, which was typically the most common variant in both biotypes. Most of the transcripts had more non-synonymous than synonymous codon changes among their variants. Our results offer possible molecular markers for distinguishing the two biotypes and insights into their evolution. en_US
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7917.2011.01487.x/pdf en_US
dc.rights This is the pre-peer-reviewed version of the following article: Cui, F., Smith, C. M., Reese, J., Edwards, O., & Reeck, G. (2012). Polymorphisms in salivary-gland transcripts of Russian wheat aphid biotypes 1 and 2. Insect Science, 19(4), 429-440, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/j.1744-7917.2011.01487.x/pdf en_US
dc.subject Biotype en_US
dc.subject Molecular marker en_US
dc.subject Polymorphism en_US
dc.subject Russian wheat aphid en_US
dc.subject Salivary gland en_US
dc.subject Transcript en_US
dc.title Polymorphisms in salivary-gland transcripts of Russian wheat aphid biotypes 1 and 2 en_US
dc.type Article (author version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:10.1111/j.1744-7917.2011.01487.x en_US
dc.citation.epage 440 en_US
dc.citation.issue 4 en_US
dc.citation.jtitle Insect Science en_US
dc.citation.spage 429 en_US
dc.citation.volume 19 en_US
dc.contributor.authoreid jreese en_US
dc.contributor.authoreid cmsmith en_US
dc.contributor.authoreid reeck en_US

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