Ocular effects following aqueocentesis in dogs using variable needle sizes: fluorophotometric and tonometric evaluation

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dc.contributor.author Allbaugh, Rachel A.
dc.date.accessioned 2009-05-14T16:43:55Z
dc.date.available 2009-05-14T16:43:55Z
dc.date.issued 2009-05-14T16:43:55Z
dc.identifier.uri http://hdl.handle.net/2097/1428
dc.description.abstract Objective – To measure blood aqueous-barrier breakdown following aqueocentesis using various needle sizes and to monitor the intraocular pressure (IOP) response. Animals – 24 healthy, adult dogs received treatment (24 treated eyes, 24 contralateral eyes); 3 dogs were untreated controls (6 control eyes). Procedures – Dogs receiving treatment were divided into 3 equal groups (25-, 27-, or 30- gauge needle aqueocentesis). In each dog the treated eye was determined randomly, the contralateral eye was untreated. Dogs that did not have aqueocentesis performed in either eye were used as controls. Aqueocentesis at the lateral limbus was performed under sedation and topical anesthesia. Anterior chamber fluorophotometry was performed before and after aqueocentesis on day 1. On days 2-5 sedation and fluorophotometry were repeated. Intraocular pressure was measured with a rebound tonometer at multiple time points. Results – Aqueocentesis resulted in blood-aqueous barrier breakdown in all treated eyes with barrier reestablishment present by day 5 detected by fluorophotometry. On day 2 the contralateral untreated eyes of all groups also showed statistically significant increased fluorescence (P < 0.05) following treatment of the opposite eye, but these values were not statistically significantly greater than untreated controls. In treated eyes there was no statistical difference in fluorescein concentration or IOP between 27- and 30-gauge needles. Use of the 25- gauge needle resulted in a statistically significant increase in anterior chamber fluorescence on days 3 and 5. It also caused a statistically significant increase in IOP 20 minutes following aqueocentesis as compared to the 27- and 30-gauge needles. Aside from this transient ocular hypertension, rapid resolution of ocular hypotony following aqueocentesis was observed in all treatment groups. Conclusions and Clinical Relevance – Aqueocentesis using a 25-gauge needle resulted in a greater degree of blood-aqueous barrier breakdown and a brief state of intraocular hypertension following paracentesis. Use of a 27- or 30-gauge needle is recommended for aqueous paracentesis. A consensual ocular reaction appeared to occur in dogs following unilateral traumatic blood-aqueous barrier breakdown and may be of clinical significance. Statistical significance was limited in this study due to high variability and large standard deviations. en
dc.description.sponsorship Kansas State University College of Veterinary Medicine Mentored Clinical, Applied or Translational Research Grant en
dc.language.iso en_US en
dc.publisher Kansas State University en
dc.subject Blood-aqueous barrier en
dc.subject Consensual ocular reaction en
dc.subject Aqueous paracentesis en
dc.subject Fluorophotometry en
dc.subject Tonometry en
dc.subject Canine en
dc.title Ocular effects following aqueocentesis in dogs using variable needle sizes: fluorophotometric and tonometric evaluation en
dc.type Thesis en
dc.description.degree Master of Science en
dc.description.level Masters en
dc.description.department Department of Clinical Sciences en
dc.description.advisor Amy J. Rankin en
dc.subject.umi Biology, Veterinary Science (0778) en
dc.subject.umi Health Sciences, Ophthalmology (0381) en
dc.date.published 2009 en
dc.date.graduationmonth May en

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