Na+ absorption by Claudius’ cells is regulated by purinergic signaling in the cochlea

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dc.contributor.author Yoo, Jae Chul
dc.contributor.author Kim, Hye-Young
dc.contributor.author Han, Kyu-Hee
dc.contributor.author Oh, Seung Ha
dc.contributor.author Chang, Sun O
dc.contributor.author Marcus, Daniel C.
dc.contributor.author Lee, Jun Ho
dc.date.accessioned 2012-07-19T15:35:57Z
dc.date.available 2012-07-19T15:35:57Z
dc.date.issued 2012-07-19
dc.identifier.uri http://hdl.handle.net/2097/14050
dc.description.abstract Conclusion: Claudius’ cells absorb Na+ through the amiloride-sensitive epithelial sodium channel (ENaC). Transepithelial ion transport through ENaC and possibly a Cl[superscript]- secretory pathway is regulated by P2Y purinergic signaling. Objectives: The purpose of this study was to investigate the ion transport in the Claudius’ cells and its purinergic regulation. Method: Young adult Sprague–Dawley rats and gerbils were studied. The Claudius’ cell layer on the basilar membrane was dissected from the basal turn of the cochlea. The voltage-sensitive vibrating probe was used to measure transepithelial short circuit current (I[subscript]sc). The baseline I[subscript]sc of Claudius’ cells was measured in the perilymph-like control solution and the change of Isc after application of amiloride (10 μM) or uridine triphosphate (UTP, 100 μM). Results: A negative baseline I[subscript]sc was observed in the control solution (-12.50 ± 3.95 μA/cm[superscript]2, n=8) and the addition of amiloride resulted in a decrease of I[subscript]sc by 75.8%. The application of UTP, an agonist for P2Y purinergic receptors, led to a partial inhibition of Isc (by 38.2 ± 3.2%, n=5), and subsequent addition of amiloride abolished the remaining I[subscript]sc. en_US
dc.relation.uri http://informahealthcare.com/doi/full/10.3109/00016489.2012.664285 en_US
dc.rights Permission to archive granted by Informa Healthcare, July 9, 2012. en_US
dc.subject Epithelial sodium channel en_US
dc.subject Purinergic receptor en_US
dc.subject Voltage-sensitive vibrating probe en_US
dc.subject Rat en_US
dc.subject Gerbil en_US
dc.title Na+ absorption by Claudius’ cells is regulated by purinergic signaling in the cochlea en_US
dc.type Article (author version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:10.3109/00016489.2012.664285 en_US
dc.citation.epage S108 en_US
dc.citation.issue S1 en_US
dc.citation.jtitle Acta Oto-Laryngologica en_US
dc.citation.spage S103 en_US
dc.citation.volume 132 en_US
dc.contributor.authoreid marcus en_US

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