Host identity impacts rhizosphere fungal communities associated with three alpine plant species

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Show simple item record Becklin, Katie M. Hertweck, Kate L. Jumpponen, Ari M. 2012-07-05T20:01:02Z 2012-07-05T20:01:02Z 2012-07-05
dc.description.abstract Fungal diversity and composition are still relatively unknown in many ecosystems; however, host identity and environmental conditions are hypothesized to influence fungal community assembly. To test these hypotheses we characterized the richness, diversity, and composition of rhizosphere fungi colonizing three alpine plant species, Taraxacum ceratophorum, Taraxacum officinale, and Polemonium viscosum. Roots were collected from open meadow and willow understory habitats at treeline on Pennsylvania Mountain, Colorado, USA. Fungal small subunit ribosomal DNA was sequenced using fungal-specific primers, sample-specific DNA tags, and 454 pyrosequencing. We classified operational taxonomic units (OTUs) as arbuscular mycorrhizal (AMF) or non-arbuscular mycorrhizal (non-AMF) fungi, then tested whether habitat or host identity influenced these fungal communities. Approximately 14% of the sequences represented AMF taxa (44 OTUs) with the majority belonging to Glomus group A and B. NONAMF sequences represented 186 OTUs belonging to Ascomycota (58%), Basidiomycota (26%), Zygomycota (14%), and Chytridiomycota (2%) phyla. Total AMF and non-AMF richness were similar between habitats, but varied among host species. AMF richness and diversity per root sample also varied among host species and were highest in T. ceratophorum compared to T. officinale and P. viscosum. In contrast, non-AMF richness and diversity per root sample were similar among host species except in the willow understory where diversity was reduced in T. officinale. Fungal community composition was influenced by host identity, but not habitat. Specifically, T. officinale hosted a different AMF community than T. ceratophorum and P. viscosum, while P. viscosum hosted a different non-AMF community than T. ceratophorum and T. officinale. Our results suggest that host identity has a stronger effect on rhizosphere fungi than habitat. Furthermore, although host identity influenced both AMF and non-AMF this effect was stronger for the mutualistic AMF community. en_US
dc.relation.uri en_US
dc.rights The final publication is available at en_US
dc.subject Host identity en_US
dc.subject Rhizosphere fungal communities en_US
dc.title Host identity impacts rhizosphere fungal communities associated with three alpine plant species en_US
dc.type Article (author version) en_US 2012 en_US
dc.citation.doi doi:10.1007/s00248-011-9968-7 en_US
dc.citation.epage 693 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle Microbial Ecology en_US
dc.citation.spage 682 en_US
dc.citation.volume 63 en_US
dc.contributor.authoreid ari en_US

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