Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo

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dc.contributor.author Liu, Qinfang
dc.contributor.author Qiao, Chuanling
dc.contributor.author Marjuki, Henju
dc.contributor.author Bawa, Bhupinder
dc.contributor.author Ma, Jingqun
dc.contributor.author Guillossou, Stephane
dc.contributor.author Webby, Richard J.
dc.contributor.author Richt, Juergen A.
dc.contributor.author Ma, Wenjun
dc.date.accessioned 2012-06-27T19:18:27Z
dc.date.available 2012-06-27T19:18:27Z
dc.date.issued 2012-06-27
dc.identifier.uri http://hdl.handle.net/2097/13965
dc.description.abstract Triple reassortant swine influenza viruses (SIVs) and 2009 pandemic H1N1 (pH1N1) virus contain an avian-origin PB2 with 271A, 590S, 591R, and 627E. To evaluate the role of PB2 271A, 590S, and 591R in the replication and virulence of SIV, single (1930-TX98-PB2-271T)-, double (1930-TX98-PB2-590A591A)-, and triple (1930-TX98-PB2-271T590A591A)-mutated viruses were generated in the background of the H1N1 A/swine/Iowa/15/30 (1930) virus with an avian-origin PB2 from the triple-reassortant A/swine/Texas/4199-2/98 (TX98) virus, called the parental 1930-TX98-PB2. Compared to parental virus and single- and double-mutated viruses, the triple-mutated virus replicated less efficiently in cell cultures and was attenuated in mice. These results suggest that a combination of 271A with the 590/591 SR polymorphism is critical for pH1N1 and triple-reassortant SIVs for efficient replication and adaptation in mammals. en_US
dc.relation.uri http://jvi.asm.org/content/86/2/1233 en_US
dc.subject Swine influenza virus en_US
dc.subject PB2 en_US
dc.subject 271A en_US
dc.subject 590/591 SR polymorphism en_US
dc.subject Replication and virulence en_US
dc.subject Cultured cells en_US
dc.subject In vivo en_US
dc.title Combination of PB2 271A and SR polymorphism at positions 590/591 is critical for viral replication and virulence of swine influenza virus in cultured cells and in vivo en_US
dc.type Article (author version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:10.1128/JVI.05699-11 en_US
dc.citation.epage 1237 en_US
dc.citation.issue 2 en_US
dc.citation.jtitle Journal of Virology en_US
dc.citation.spage 1233 en_US
dc.citation.volume 86 en_US
dc.contributor.authoreid jricht en_US
dc.contributor.authoreid bbawa en_US
dc.contributor.authoreid mjqo819 en_US
dc.contributor.authoreid sguill en_US
dc.contributor.authoreid wjma en_US

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