Genome organization, phylogenies, expression patterns, and three-dimensional protein models of two acetylcholinesterase genes from the red flour beetle

K-REx Repository

Show simple item record

dc.contributor.author Lu, Yanhui
dc.contributor.author Pang, Yuan-Ping
dc.contributor.author Park, Yoonseong
dc.contributor.author Gao, Xiwu
dc.contributor.author Yao, Jianxiu
dc.contributor.author Zhang, Xin
dc.contributor.author Zhu, Kun Yan
dc.date.accessioned 2012-05-23T15:48:37Z
dc.date.available 2012-05-23T15:48:37Z
dc.date.issued 2012-05-23
dc.identifier.uri http://hdl.handle.net/2097/13852
dc.description.abstract Since the report of a paralogous acetylcholinesterase (AChE, EC3.1.1.7) gene in the greenbug (Schizaphis graminum) in 2002, two different AChE genes (Ace1 and Ace2) have been identified in each of at least 27 insect species. However, the gene models of Ace1 and Ace2, and their molecular properties have not yet been comprehensively analyzed in any insect species. In this study, we sequenced the full-length cDNAs, computationally predicted the corresponding three-dimensional protein models, and profiled developmental stage and tissue-specific expression patterns of two Ace genes from the red flour beetle (Tribolium castaneum; TcAce1 and TcAce2), a globally distributed major pest of stored grain products and an emerging model organism. TcAce1 and TcAce2 encode 648 and 604 amino acid residues, respectively, and have conserved motifs including a choline-binding site, a catalytic triad, and an acyl pocket. Phylogenetic analysis show that both TcAce genes are grouped into two insect Ace clusters and TcAce1 is completely diverged from TcAce2, suggesting that these two genes evolve from their corresponding Ace gene lineages in insect species. In addition, TcAce1 is located on chromosome 5, whereas TcAce2 is located on chromosome 2. Reverse transcription polymerase chain reaction (PCR) and quantitative realtime PCR analyses indicate that both genes are virtually transcribed in all the developmental stages and predominately expressed in the insect brain. Our computational analyses suggest that the TcAce1 protein is a robust acetylcholine (ACh) hydrolase and has susceptibility to sulfhydryl agents whereas the TcAce2 protein is not a catalytically efficient ACh hydrolase. en_US
dc.relation.uri http://www.plosone.org/article/info%253Adoi%252F10.1371%252Fjournal.pone.0032288 en_US
dc.subject Acetylcholinesterase en_US
dc.subject Red flour beetle en_US
dc.title Genome organization, phylogenies, expression patterns, and three-dimensional protein models of two acetylcholinesterase genes from the red flour beetle en_US
dc.type Article (publisher version) en_US
dc.date.published 2012 en_US
dc.citation.doi doi:10.1371/journal.pone.0032288 en_US
dc.citation.epage e32288 en_US
dc.citation.issue 2 en_US
dc.citation.jtitle PLoS ONE en_US
dc.citation.spage e32288 en_US
dc.citation.volume 7 en_US
dc.contributor.authoreid xz65 en_US
dc.contributor.authoreid ypark en_US
dc.contributor.authoreid jianxiuy en_US
dc.contributor.authoreid kzhu en_US

Files in this item


Files Size Format View

This item appears in the following Collection(s)

Show simple item record