Val[a]idating the efficacy of commercial foaming cleaner and sanitizer for controlling Listeria innocua (surrogate for Listeria monocytogenes) in drains and potential translocation from the drain to the food contact surfaces

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dc.contributor.author Saini, Jasdeep Kaur
dc.date.accessioned 2008-12-16T20:33:28Z
dc.date.available 2008-12-16T20:33:28Z
dc.date.issued 2008-12-16T20:33:28Z
dc.identifier.uri http://hdl.handle.net/2097/1061
dc.description.abstract Listeria monocytogenes is known to be an environmental contaminant in food processing facilities. Floor drains in processing environments harbor Listeria spp. due to continuous presence of humidity and organic substrates. The cleaning and washing activities undertaken may translocate the bacterial cells from the drain to the surrounding environment, thus contaminating food products being produced. This study validates the effectiveness of Johnson Diversey ‘Eliminex’ Foaming Drain Cleaner and Johnson Diversey ‘Final Step’ 512 sanitizer for inhibition of Listeria monocytogenes in drain surfaces and evaluates the potential for translocation of L. monocytogenes from drains to food contact surfaces in the surrounding environment using Listeria innocua as a surrogate. A 7x 7 x 8 feet flexi glass chamber was built in which a 10 inch diameter drain mounted on an aluminum cabinet was placed. The drain was inoculated with the surrogate organism, L. innocua, at specific time intervals and then treated with the given chemicals. Sponge samples were taken and bacterial populations were recovered on Tryptic Soy Agar (TSA), Modified Oxford Medium (MOX) and Thin Agar Layer MOX (TALMOX). Stainless steel coupons (6.4 x 1.9 x 0.1 cm) were hung at 3 different heights 1, 3 and 5 feet inside the chamber and cell translocation from the drain on to the stainless steel coupons was studied. Reductions up to 4 Log CFU/area or ml were seen at the drain surface, drain crate, drain pipe and wash water for both free cells and cells entrapped in biofilms Treatment had a significant effect (p<0.05) on the reduction of bacterial cells. The wash water showed the greatest reduction from 8 Log CFU/ml to est. 0.23 Log CFU/ml. The given cleaner and sanitizer were found to be effective for reducing Listeria spp. on drain surfaces. Results for the second part indicated translocation at all three heights with percentage translocation ranging between 2-17%. Significantly higher translocation (p<0.05) was seen at 1 foot, followed by 3 feet and 5 feet indicating the closer the height to the drain, the greater the number of bacterial cells that are able to transfer from the drain to the surrounding environment. en
dc.language.iso en_US en
dc.publisher Kansas State University en
dc.subject Listeria monocytogenes en
dc.subject Listeria innocua en
dc.subject Drains en
dc.subject Food contact surfaces en
dc.title Val[a]idating the efficacy of commercial foaming cleaner and sanitizer for controlling Listeria innocua (surrogate for Listeria monocytogenes) in drains and potential translocation from the drain to the food contact surfaces en
dc.title.alternative Validating the efficacy of commercial foaming cleaner and sanitizer for controlling Listeria innocua (surrogate for Listeria monocytogenes) in drains and potential translocation from the drain to the food contact surfaces en
dc.type Thesis en
dc.description.degree Master of Science en
dc.description.level Masters en
dc.description.department Food Science Institute en
dc.description.advisor Daniel Y.C. Fung en
dc.description.advisor James L. Marsden en
dc.subject.umi Agriculture, Food Science and Technology (0359) en
dc.date.published 2008 en
dc.date.graduationmonth December en

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