http://hdl.handle.net/2097/1063 Search the Channel search http://krex.ksu.edu/dspace/simple-search http://hdl.handle.net/2097/2075 Title: MPH Field Experience<br/><br/>Authors: McGill, Zachary<br/><br/>Abstract: The following documents are a collection of products finished by Kansas State University graduate student Zachary McGill in support for his master’s degree in public health. The student had a field experience at three different sites; the Kansas Health Institute, the Kansas Public Health Association, and the Network for a Healthy California, Los Angeles Region. The student was able to gain experience in by working on public health, community events, grant writing, article writing, research, and policy at all three sites. http://hdl.handle.net/2097/1685 Title: Investigation of intestinal parasitism among Hispanic migrant and seasonal farm workers in eastern North Carolina<br/><br/>Authors: Russell, Mindi<br/><br/>Abstract: In collaboration between the North Carolina State Laboratory of Public Health (NCSLPH) and Dr. Maria T. Correa from the North Carolina State University College of Veterinary Medicine, the field experience focused on the development and application of accurate and rapid methods to detect and generally characterize Taenia solium in human stool samples. T. solium, the "pork tapeworm," is associated with cysticercosis in humans, is distributed worldwide, and the leading cause of adult-onset epilepsy. Approximately 90% of patients with cysticercosis develop nuerocysticercosis (NCC). NCC is endemic in most of Central and South America, and Asia. In the U.S., over 1,000 NCC cases are diagnosed each year, mostly in the immigrant population. In North Carolina, 192 cases of NCC have been diagnosed between 1995 and 2002. In initial studies, Multiplex PCR was used for the differential diagnosis of taeniasis, caused by T. saginate, T. asiatica and two genotypes of T. solium (Asian and American/African genotypes). This detection approach was evaluated on clinical (fecal) specimens submitted to NCSLPH for diagnostic purposes with the intent to expand screening to include a larger percentage of the immigrant populations. http://hdl.handle.net/2097/1639 Title: Comparative in vitro estimates of inhalation toxicity of selected nanoparticles<br/><br/>Authors: Dhakal, Kiran<br/><br/>Abstract: Airway inflammation is characterized by the release of pro-inflammatory cytokines (IL-6) and chemokine (IL-8) from airway epithelial cells. To screen for the potential inhalation toxicity as inflammation, we tested exposure of metal oxide nanoparticles (NPs)-Titanium dioxide, Magnesium oxide, FastAct and Titanium Silicon Oxide-Manganese (TSO-Mn)-Aerogel to BEAS 2B human bronchial epithelial cells. A monolayer of cells having 80 – 90% confluence was treated with different concentrations of the NPs and feedlot dust as positive control for inflammatory processes. Releases of IL-6 and IL-8 into the culture supernatant fluid were measured by sandwich enzyme-linked immunoassay (ELISA). Characterization of NPs such as solubility and agglomeration in cell culture media were carried out to predict the effect of these properties in cellular responses. Feedlot dust increased the release of both IL-6 and IL-8 by 3 to >5 fold, suggesting an inflammatory effect while NPs did not show any effect either at increasing the dose or duration of the incubation with cell. The NPs at higher doses reduced the total IL-6 and IL-8 released, suggesting that the NPs may have bound with the cytokine and chemokine or somehow interfered with their function. The inert activity of NPs was further investigated by inspecting cell morphology, counting viable cells and assessing mitochondrial membrane potential. Concentrations at 1000 mg/L of TiO2 and 250 mg/L TSO-Mn-Aerogel could apparently limit lung epithelial cell multiplication by partially occupying the intercellular spaces, qualitatively increasing the number of cell pores and resulting in less recovered cells after 12 hours of incubation. Cells exposed to feedlot dust and titanium NPs were less viable as indicated by propidium iodide staining, but cells exposed to TSO-Mn-aerogel were more apoptotic as indicated JC-1 staining. These changes occurred at projected inhalation exposure levels > 40-100 fold above the nuisance dust level for TiO2 and permissible exposure limit for Manganese. No MgO exposures reduced apparently recovered cells to < 50% as indicated by manual hemocytometer counts (+ 15-25% variability). The lack of toxicity was most likely reflected from the high MgO solubility in the incubating media, and the relative non-toxicity of MgO. http://hdl.handle.net/2097/1307 Title: Army public health and preventive medicine: Proactive approaches to readiness<br/><br/>Authors: Cates, Michael B<br/><br/>Abstract: Preventive medicine is crucial in maintaining the readiness of our most important resource—our people. Sustaining, and even improving, a Soldier’s health is a much wiser use of resources than waiting until that Soldier becomes sick or injured before attempting torestore health. The better we prevent diseases,conditions and injuries, the more resources will be available to apply to those things we cannot prevent. While there is continuing and growing emphasis on proactive approaches to health in today’s society and military, we must all strive toward translating that into real, even greater long-term nvestments in the future of our personnel. Prevention is the best way to health.